0301 Does Orexin Influence Sleep Fragmented Brain Inflammation? A Pilot Study

Abstract Introduction Fragmented sleep occurs when there are repetitive, short interruptions of sleep, resulting in less than six hours per day. Overall, however, the United States, fragmentation is reported by 30% employed adults. Sleep may be a risk factor for Alzheimer's disease, and orexin reduction appears effective reducing amyloid plaque formation mice with transgenic disease. Orexin neuropeptide that regulates arousal, wakefulness, appetite. Thus we hypothesized loss might also protective against disruption injury non-Alzheimer's mice. This study aims to combine see if brain health severely reduced. Methods The 24 this were split into four groups: B6 rested, fragmented, knockout fragmented. fragmented placed on shaker table 10½ weeks initiate chronic loss. all perfused within five eight months birth, then brains cryopreserved sliced. These sections immunolabeled different protein antibodies using immunohistochemistry techniques. stained either analyzed through microscope stereology counts or computer image analysis. Results Two-way ANOVA analysis tyrosine hydroxylase, ionized calcium binding adaptor, vesicular acetylcholine transporter had p<0.05 variable, showing differences these rested cluster differentiation 68, cofilin, postsynaptic density protein, RanBP genotype normal glial fibrillary acidic amyloid-beta both variables, levels. There was no significance synapsin. Conclusion Our results show knocking out orexinergic neurons causes hippocampal tissue damage, dampens functioning synapses, diminishes ability adapt plasticity memory. fragmentation, increases phagocytic activity, harms norepinepherine neurotransmitter pathways. When combined, cell communication worsens blood barrier loses function, resemblant neurodegenerative diseases. Support (If Any) NIH AG054104; AG064231